Chemoprevention of dmba-induced mammary gland carcinogenesis--preventive effects of free oxygen radical scavengers.

ABSTRACT: This study was carried out to determine the effects of Mangan-Desferrioxamine (Mn-DFX) and Verapamil (VRP) in 7,12-dimethyl-benz-[a]anthracene (DMBA) induced mammary carcinoma. 70 Spraque Dawley rats were divided into four groups; as DMBA alone, DMBA + Mn-DFX, DMBA + VRP, and control. Incidence, multiplicity, and size of tumors were evaluated in addition to analyses of DNA ploidy status and proliferation index by flow cytometric technique. The results revealed that Mn-DFX and VRP caused significant decreases in tumor incidence (p < 0.02 and p < 0.05), multiplicity (p < 0.02 and p < 0.05), size (p < 0.05 and p < 0.02), and proliferation index (p < 0.05 and p < 0.02) without any toxic effect. The appearance of first tumors in Mn-DFX and VRP groups was also later than in the DMBA alone group (10th and 11th week versus 6th week). In conclusion, Mn-DFX and VRP have offered prevention in experimental mammary carcinogenesis. These agents caused slower tumor growth, though they could not achieve a complete prevention.

G Soybir, F Köksoy, H Koyuncu, O Yalçin, H Köse, C Topuzlu

Breast Cancer Research and Treatment 08/1998; 50(2):193-9